Publications

2021年

  1. Paudel, A., Panthee, S., Hamamoto, H., Grunert, T., and Sekimizu, K. (2021) YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus. Virulence 12, 470-480
  2. Panthee, S., Paudel, A., Hamamoto, H., Ogasawara, A. A., Iwasa, T., Blom, J., and Sekimizu, K. (2021) Complete genome sequence and comparative genomic analysis of Enterococcus faecalis EF-2001, a probiotic bacterium. Genomics 113, 1534-1542
  3. Nakajima, H., Miyashita, A., Hamamoto, H., and Sekimizu, K. (2021) A novel application of bubble-eye strain of Carassius auratus for ex vivo fish immunological studies. Sci. Rep. 11, 10757
  4. Miyashita, A., Shimomura, K., Nakajima, H., Matsumoto, Y., and Sekimizu, K. (2021) A digital scheme of human trials for the evaluation of functional foods. Drug Discov. Ther. 15, 9-13
  5. Miyashita, A., and Sekimizu, K. (2021) Using silkworms to search for lactic acid bacteria that contribute to infection prevention and improvement of hyperglycemia. Drug Discov. Ther.
  6. Miyashita, A., Kataoka, K., Tsuchida, T., Ogasawara, A. A., Hamamoto, H., Takahashi, M., and Sekimizu, K. (2021) Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica). bioRxiv, 2021.2005.2026.445781
  7. Miyashita, A., Kataoka, K., and Sekimizu, K. (2021) Activation of innate immunity by neutral polysaccharides from broccoli buds. bioRxiv
  8. Mikami, K., Sonobe, K., Ishino, K., Noda, T., Kato, M., Hanao, M., Hamamoto, H., Sekimizu, K., and Okazaki, M. (2021) Evaluation of pathogenicity and therapeutic effectiveness of antibiotics using silkworm Nocardia infection model. Drug Discov. Ther. 15, 73-77
  9. Miyashita, A., Hamamoto, H., and Sekimizu, K. Applying the silkworm model for the search of immunosuppressants. Drug Discov. Ther. In press

2020年以前

  1. Takada, Y., Itoh, H., Paudel, A., Panthee, S., Hamamoto, H., Sekimizu, K., and Inoue, M. (2020) Discovery of gramicidin A analogues with altered activities by multidimensional screening of a one-bead-one-compound library. Nature Communications 11, 4935
  2. Paudel, A., Panthee, S., Hamamoto, H., and Sekimizu, K. (2020) A simple artificial diet available for research of silkworm disease models. Drug Discov. Ther. 14, 177-180
  3. Paudel, A., Hamamoto, H., Panthee, S., Matsumoto, Y., and Sekimizu, K. (2020) Large-Scale Screening and Identification of Novel Pathogenic Staphylococcus aureus Genes Using a Silkworm Infection Model. J. Infect. Dis. 221, 1795-1804
  4. Panthee, S., Paudel, A., Hamamoto, H., Uhlemann, A.-C., and Sekimizu, K. (2020) The Role of Amino Acid Substitution in HepT Toward Menaquinone Isoprenoid Chain Length Definition and Lysocin E Sensitivity in Staphylococcus aureus. Front. Microbiol. 11
  5. Matsumoto, Y., Takahashi, M., and Sekimizu, K. (2020) Polysaccharides of a fermented food, natto, suppress sucrose-induced hyperglycemia in an in vivo evaluation system and inhibit glucose uptake by human intestinal cells. Drug Discov. Ther. 14, 8-13
  6. Matsumoto, Y., Takahashi, M., and Sekimizu, K. (2020) Polysaccharides of a fermented food, natto, suppress sucrose-induced hyperglycemia in an in vivo evaluation system and inhibit glucose uptake by human intestinal cells. Drug Discov. Ther. 14, 8-13
  7. Matsumoto, Y., and Sekimizu, K. (2020) Silkworm Infection Model for Evaluating Pathogen Virulence. in Immunity in Insects, Springer. pp 233-240
  8. Kaito, C., Yoshikai, H., Wakamatsu, A., Miyashita, A., Matsumoto, Y., Fujiyuki, T., Kato, M., Ogura, Y., Hayashi, T., Isogai, T., and Sekimizu, K. (2020) Non-pathogenic Escherichia coli acquires virulence by mutating a growth-essential LPS transporter. PLoS Pathog. 16, e1008469
  9. Kaito, C., Yoshikai, H., Wakamatsu, A., Miyashita, A., Matsumoto, Y., Fujiyuki, T., Kato, M., Ogura, Y., Hayashi, T., and Isogai, T. (2020) Non-pathogenic Escherichia coli acquires virulence by mutating a growth-essential LPS transporter. PLoS Pathog. 16, e1008469
  10. Ryuno, H., Nigo, F., Naguro, I., Sekimizu, K., and Kaito, C. (2019) Staphylococcus aureus aggregation in the plasma fraction of silkworm hemolymph. PLoS One 14, e0217517
  11. Paudel, A., Panthee, S., Hamamoto, H., and Sekimizu, K. (2019) GPI0363 inhibits the interaction of RNA polymerase with DNA in Staphylococcus aureus. RSC Advances 9, 37889-37894
  12. Panthee, S., Paudel, A., Blom, J., Hamamoto, H., and Sekimizu, K. (2019) Complete Genome Sequence of Weissella hellenica 0916-4-2 and Its Comparative Genomic Analysis. Front. Microbiol. 10, 1619
  13. Nwibo, D. D., Panthee, S., Hamamoto, H., and Sekimizu, K. (2019) Molecular characterization of multi-drug resistant coagulase negative cocci in non-hospital environment. Drug Discov. Ther. 13, 145-149
  14. Mitsutomi, S., Akimitsu, N., Sekimizu, K., and Kaito, C. (2019) Identification of 2H phosphoesterase superfamily proteins with 2′-CPDase activity. Biochimie 165, 235-244
  15. Matsumoto, Y., Takahashi, M., Umehara, M., Asano, M., Maruki-Uchida, H., Morita, M., and Sekimizu, K. (2019) Suppressive effects of whey protein hydrolysate on sucrose-induced hyperglycemia in silkworms. Drug Discov. Ther. 13, 244-247
  16. Matsumoto, Y., Ishii, M., Hasegawa, S., and Sekimizu, K. (2019) Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans. Commun Biol 2, 157
  17. Itoh, H., Tokumoto, K., Kaji, T., Paudel, A., Panthee, S., Hamamoto, H., Sekimizu, K., and Inoue, M. (2019) Development of a high-throughput strategy for discovery of potent analogues of antibiotic lysocin E. Nat Commun 10, 2992
  18. Ishijima, S. A., Hiruma, M., Sekimizu, K., and Abe, S. (2019) Detection of Trichophyton spp. from footwear of patients with tinea pedis. Drug Discov. Ther. 13, 207-211
  19. Ishii, M., Matsumoto, Y., Katada, T., and Sekimizu, K. (2019) Additive effects of Kothala himbutu (Salacia reticulata) extract and a lactic acid bacterium (Enterococcus faecalis YM0831) for suppression of sucrose-induced hyperglycemia in an in vivo silkworm evaluation system. Drug Discov. Ther. 13, 133-136
  20. Hamamoto, H., Horie, R., and Sekimizu, K. (2019) Pharmacokinetics of anti-infectious reagents in silkworms. Sci. Rep. 9, 9451
  21. Paudel, A., Panthee, S., Urai, M., Hamamoto, H., Ohwada, T., and Sekimizu, K. (2018) Pharmacokinetic parameters explain the therapeutic activity of antimicrobial agents in a silkworm infection model. Sci. Rep. 8, 1578
  22. Panthee, S., Hamamoto, H., Ishijima, S. A., Paudel, A., and Sekimizu, K. (2018) Utilization of Hybrid Assembly Approach to Determine the Genome of an Opportunistic Pathogenic Fungus, Candida albicans TIMM 1768. Genome Biol. Evol. 10, 2017-2022
  23. Nakajima, H., Matsumoto, Y., and Sekimizu, K. (2018) Establishment of a gnotobiotic silkworm model. Drug Discov. Ther. 12, 291-294
  24. Maruyama, T., Baba, T., Maemoto, Y., Hara-Miyauchi, C., Hasegawa-Ogawa, M., Okano, H. J., Enda, Y., Matsumoto, K., Arimitsu, N., Nakao, K., Hamamoto, H., Sekimizu, K., Ohto-Nakanishi, T., Nakanishi, H., Tokuyama, T., Yanagi, S., Tagaya, M., and Tani, K. (2018) Loss of DDHD2, whose mutation causes spastic paraplegia, promotes reactive oxygen species generation and apoptosis. Cell Death Dis. 9, 797
  25. Ishii, M., Matsumoto, Y., and Sekimizu, K. (2018) Estimation of lactic acid bacterial cell number by DNA quantification. Drug Discov. Ther. 12, 88-91
  26. Ishii, M., Matsumoto, Y., and Sekimizu, K. (2018) Inhibitory effects of alpha-cyclodextrin and its derivative against sucrose-induced hyperglycemia in an in vivo evaluation system. Drug Discov. Ther. 12, 122-125
  27. Ishii, M., Matsumoto, Y., and Sekimizu, K. (2018) Bacterial polysaccharides inhibit sucrose-induced hyperglycemia in silkworms. Drug Discov. Ther. 12, 185-188
  28. Yagi, A., Uchida, R., Hamamoto, H., Sekimizu, K., Kimura, K. I., and Tomoda, H. (2017) Anti-Mycobacterium activity of microbial peptides in a silkworm infection model with Mycobacterium smegmatis. J. Antibiot. (Tokyo) 70, 685-690
  29. Urai, M., Kataoka, K., Nishida, S., and Sekimizu, K. (2017) Structural analysis of an innate immunostimulant from broccoli, Brassica oleracea var. italica. Drug Discov. Ther. 11, 230-237
  30. Urai, M., Aizawa, T., Imamura, K., Hamamoto, H., and Sekimizu, K. (2017) Characterization of the chemical structure and innate immune-stimulating activity of an extracellular polysaccharide from Rhizobium sp. strain M2 screened using a silkworm muscle contraction assay. Drug Discov. Ther. 11, 238-245
  31. Tabuchi, F., Matsumoto, Y., Ishii, M., Tatsuno, K., Okazaki, M., Sato, T., Moriya, K., and Sekimizu, K. (2017) D-cycloserine increases the effectiveness of vancomycin against vancomycin-highly resistant Staphylococcus aureus. J. Antibiot. (Tokyo) 70, 907-910
  32. Tabuchi, F., Matsumoto, Y., Ishii, M., Tatsuno, K., Okazaki, M., Sato, T., Moriya, K., and Sekimizu, K. (2017) Synergistic effects of vancomycin and beta-lactams against vancomycin highly resistant Staphylococcus aureus. J. Antibiot. (Tokyo)
  33. Paudel, A., Hamamoto, H., Panthee, S., Kaneko, K., Matsunaga, S., Kanai, M., Suzuki, Y., and Sekimizu, K. (2017) A Novel Spiro-Heterocyclic Compound Identified by the Silkworm Infection Model Inhibits Transcription in Staphylococcus aureus. Front. Microbiol. 8, 712
  34. Panthee, S., Paudel, A., Hamamoto, H., and Sekimizu, K. (2017) Advantages of the Silkworm As an Animal Model for Developing Novel Antimicrobial Agents. Front. Microbiol. 8, 373
  35. Panthee, S., Paudel, A., Hamamoto, H., and Sekimizu, K. (2017) Draft Genome Sequence of the Vancomycin-Resistant Clinical Isolate Staphylococcus aureus VRS3b. Genome Announc 5, e00452-00417
  36. Panthee, S., Hamamoto, H., Suzuki, Y., and Sekimizu, K. (2017) In silico identification of lysocin biosynthetic gene cluster from Lysobacter sp. RH2180-5. J. Antibiot. (Tokyo) 70, 204-207
  37. Panthee, S., Hamamoto, H., Paudel, A., and Sekimizu, K. (2017) Genomic analysis of vancomycin-resistant Staphylococcus aureus VRS3b and its comparison with other VRSA isolates. Drug Discov. Ther. 11, 78-83
  38. Nishida, S., Ishii, M., Nishiyama, Y., Abe, S., Ono, Y., and Sekimizu, K. (2017) Lactobacillus paraplantarum 11-1 Isolated from Rice Bran Pickles Activated Innate Immunity and Improved Survival in a Silkworm Bacterial Infection Model. Front. Microbiol. 8, 436
  39. Nakamura, I., Kanasaki, R., Yoshikawa, K., Furukawa, S., Fujie, A., Hamamoto, H., and Sekimizu, K. (2017) Discovery of a new antifungal agent ASP2397 using a silkworm model of Aspergillus fumigatus infection. J. Antibiot. (Tokyo) 70, 41-44
  40. Mitsutomi, S., Sekimizu, K., and Kaito, C. (2017) Isolation of antibiotic-producing Pseudomonas species with low-temperature cultivation of temperate soil. Drug Discov. Ther. 11, 267-275
  41. Maruki-Uchida, H., Sai, M., and Sekimizu, K. (2017) Evaluation of the innate immune-stimulating activity of amazake using a silkworm muscle contraction assay. Drug Discov. Ther. 11, 288-290
  42. Kochi, Y., Matsumoto, Y., Sekimizu, K., and Kaito, C. (2017) Two-spotted cricket as an animal infection model of human pathogenic fungi. Drug Discov. Ther. 11, 259-266
  43. Kheawfu, K., Pikulkaew, S., Hamamoto, H., Sekimizu, K., and Okonogi, S. (2017) Influence of clove oil and eugenol on muscle contraction of silkworm (Bombyx mori). Drug Discov. Ther. 11, 64-69
  44. Itoh, H., Tokumoto, K., Kaji, T., Paudel, A., Panthee, S., Hamamoto, H., Sekimizu, K., and Inoue, M. (2017) Total Synthesis and Biological Mode of Action of WAP-8294A2: A Menaquinone-Targeting Antibiotic. J. Org. Chem. 83, 6924-6935
  45. Ishii, M., Nishida, S., Kataoka, K., Nishiyama, Y., Abe, S., and Sekimizu, K. (2017) Lactic acid bacteria of the Leuconostoc genus with high innate immunity-stimulating activity. Drug Discov. Ther. 11, 25-29
  46. Ishii, M., Matsumoto, Y., Yamada, T., Abe, S., and Sekimizu, K. (2017) An invertebrate infection model for evaluating anti-fungal agents against dermatophytosis. Sci. Rep. 7, 12289
  47. Ishii, M., Matsumoto, Y., Nishida, S., and Sekimizu, K. (2017) Decreased sugar concentration in vegetable and fruit juices by growth of functional lactic acid bacteria. Drug Discov. Ther. 11, 30-34
  48. Fujino, H., Nagatomo, M., Paudel, A., Panthee, S., Hamamoto, H., Sekimizu, K., and Inoue, M. (2017) Unified Total Synthesis of Polyoxins J, L, and Fluorinated Analogues on the Basis of Decarbonylative Radical Coupling Reactions. Angew. Chem. Int. Ed. Engl. 56, 11865-11869
  49. Anantaworasakul, P., Hamamoto, H., Sekimizu, K., and Okonogi, S. (2017) Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract. Drug Discov. Ther. 11, 70-77
  50. Anantaworasakul, P., Hamamoto, H., Sekimizu, K., and Okonogi, S. (2017) In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms. Pharm. Biol. 55, 1256-1262
  51. Yoshikai, H., Kizaki, H., Saito, Y., Omae, Y., Sekimizu, K., and Kaito, C. (2016) Multidrug-Resistance Transporter AbcA Secretes Staphylococcus aureus Cytolytic Toxins. J. Infect. Dis. 213, 295-304
  52. Yamamoto, M., Kashimoto, T., Yoshimura, Y., Tachibana, N., Kuroda, S., Miki, Y., Kitabayashi, S., Tong, P., Xiao, J., Tanaka, K., Hamamoto, H., Sekimizu, K., and Yamamoto, K. (2016) A silkworm infection model to investigate Vibrio vulnificus virulence genes. Mol Med Rep 14, 4243-4247
  53. Usui, K., Nishida, S., Sugita, T., Ueki, T., Matsumoto, Y., Okumura, H., and Sekimizu, K. (2016) Acute oral toxicity test of chemical compounds in silkworms. Drug Discov. Ther. 10, 57-61
  54. Paudel, A., Hamamoto, H., Panthee, S., and Sekimizu, K. (2016) Menaquinone as a potential target of antibacterial agents. Drug Discov. Ther. 10, 123-128
  55. Nishida, S., Ono, Y., and Sekimizu, K. (2016) Lactic acid bacteria activating innate immunity improve survival in bacterial infection model of silkworm. Drug Discov. Ther. 10, 49-56
  56. Nakadai, M., Tomida, S., and Sekimizu, K. (2016) An Intriguing Correlation Based on the Superimposition of Residue Pairs with Inhibitors that Target Protein-Protein Interfaces. Sci. Rep. 6, 18543
  57. Miyashita, A., Kizaki, H., Sekimizu, K., and Kaito, C. (2016) Body-enlarging effect of royal jelly in a non-holometabolous insect species, Gryllus bimaculatus. Biol Open 5, 770-776
  58. Miyashita, A., Kizaki, H., Sekimizu, K., and Kaito, C. (2016) No Effect of Body Size on the Frequency of Calling and Courtship Song in the Two-Spotted Cricket, Gryllus bimaculatus. PLoS One 11, e0146999
  59. Matsumoto, Y., Yasukawa, J., Ishii, M., Hayashi, Y., Miyazaki, S., and Sekimizu, K. (2016) A critical role of mevalonate for peptidoglycan synthesis in Staphylococcus aureus. Sci. Rep. 6, 22894
  60. Matsumoto, Y., Ishii, M., and Sekimizu, K. (2016) An in vivo invertebrate evaluation system for identifying substances that suppress sucrose-induced postprandial hyperglycemia. Sci. Rep. 6, 26354
  61. Kochi, Y., Miyashita, A., Tsuchiya, K., Mitsuyama, M., Sekimizu, K., and Kaito, C. (2016) A human pathogenic bacterial infection model using the two-spotted cricket, Gryllus bimaculatus. FEMS Microbiol. Lett. 363, fnw163
  62. Kizaki, H., Omae, Y., Tabuchi, F., Saito, Y., Sekimizu, K., and Kaito, C. (2016) Cell-Surface Phenol Soluble Modulins Regulate Staphylococcus aureus Colony Spreading. PLoS One 11, e0164523
  63. Kaji, T., Murai, M., Itoh, H., Yasukawa, J., Hamamoto, H., Sekimizu, K., and Inoue, M. (2016) Total Synthesis and Functional Evaluation of Fourteen Derivatives of Lysocin E: Importance of Cationic, Hydrophobic, and Aromatic Moieties for Antibacterial Activity. Chemistry (Easton) 22, 16912-16919
  64. Inagaki, Y., Matsumoto, Y., Tang, W., and Sekimizu, K. (2016) Dividing phase-dependent cytotoxicity profiling of human embryonic lung fibroblast identifies candidate anticancer reagents. Drug Discov. Ther. 10, 195-200
  65. Imae, K., Saito, Y., Kizaki, H., Ryuno, H., Mao, H., Miyashita, A., Suzuki, Y., Sekimizu, K., and Kaito, C. (2016) Novel Nucleoside Diphosphatase Contributes to Staphylococcus aureus Virulence. J. Biol. Chem. 291, 18608-18619
  66. Su, J., Iehara, M., Yasukawa, J., Matsumoto, Y., Hamamoto, H., and Sekimizu, K. (2015) A novel mutation in the vraS gene of Staphylococcus aureus contributes to reduce susceptibility against daptomycin. J. Antibiot. (Tokyo) 68, 646-648
  67. Nwibo, D. D., Matsumoto, Y., and Sekimizu, K. (2015) Identification and methods for prevention of Enterococcus mundtii infection in silkworm larvae, Bombyx mori, reared on artificial diet. Drug Discov. Ther. 9, 184-190
  68. Murai, M., Kaji, T., Kuranaga, T., Hamamoto, H., Sekimizu, K., and Inoue, M. (2015) Total synthesis and biological evaluation of the antibiotic lysocin e and its enantiomeric, epimeric, and N-demethylated analogues. Angew. Chem. Int. Ed. Engl. 54, 1556-1560
  69. Miyashita, A., Takahashi, S., Ishii, K., Sekimizu, K., and Kaito, C. (2015) Primed Immune Responses Triggered by Ingested Bacteria Lead to Systemic Infection Tolerance in Silkworms. PLoS One 10, e0130486
  70. Miyashita, A., Hirai, Y., Sekimizu, K., and Kaito, C. (2015) Antibiotic-producing bacteria from stag beetle mycangia. Drug Discov. Ther. 9, 33-37
  71. Matsumoto, Y., Ishii, M., Hayashi, Y., Miyazaki, S., Sugita, T., Sumiya, E., and Sekimizu, K. (2015) Diabetic silkworms for evaluation of therapeutically effective drugs against type II diabetes. Sci. Rep. 5, 10722
  72. Mao, J., Kuranaga, T., Hamamoto, H., Sekimizu, K., and Inoue, M. (2015) Rational design, synthesis, and biological evaluation of lactam-bridged gramicidin a analogues: discovery of a low-hemolytic antibacterial Peptide. ChemMedChem 10, 540-545
  73. Kyuma, T., Kizaki, H., Ryuno, H., Sekimizu, K., and Kaito, C. (2015) 16S rRNA methyltransferase KsgA contributes to oxidative stress resistance and virulence in Staphylococcus aureus. Biochimie
  74. Kyuma, T., Kimura, S., Hanada, Y., Suzuki, T., Sekimizu, K., and Kaito, C. (2015) Ribosomal RNA methyltransferases contribute to Staphylococcus aureus virulence. FEBS J 282, 2570-2584
  75. Ishii, M., Matsumoto, Y., and Sekimizu, K. (2015) Compounds in a particular production lot of tryptic soy broth inhibit Staphylococcus aureus cell growth. Drug Discov. Ther. 9, 178-183
  76. Ishii, K., Tabuchi, F., Matsuo, M., Tatsuno, K., Sato, T., Okazaki, M., Hamamoto, H., Matsumoto, Y., Kaito, C., Aoyagi, T., Hiramatsu, K., Kaku, M., Moriya, K., and Sekimizu, K. (2015) Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis. Sci. Rep. 5, 17092
  77. Ishii, K., Hamamoto, H., and Sekimizu, K. (2015) Paralytic Peptide: an insect cytokine that mediates innate immunity. Arch. Insect Biochem. Physiol. 88, 18-30
  78. Inagaki, Y., Matsumoto, Y., Ishii, M., Uchino, K., Sezutsu, H., and Sekimizu, K. (2015) Fluorescence imaging for a noninvasive in vivo toxicity-test using a transgenic silkworm expressing green fluorescent protein. Sci. Rep. 5, 11180
  79. Hamamoto, H., Urai, M., Ishii, K., Yasukawa, J., Paudel, A., Murai, M., Kaji, T., Kuranaga, T., Hamase, K., Katsu, T., Su, J., Adachi, T., Uchida, R., Tomoda, H., Yamada, M., Souma, M., Kurihara, H., Inoue, M., and Sekimizu, K. (2015) Lysocin E is a new antibiotic that targets menaquinone in the bacterial membrane. Nat. Chem. Biol. 11, 127-133
  80. Adachi, T., Matsumoto, Y., Inagaki, Y., and Sekimizu, K. (2015) Niemann-Pick disease type C2 protein induces autophagy and inhibits growth in FM3A breast cancer cells. Drug Discov. Ther. 9, 282-288
  81. Uchida, R., Hanaki, H., Matsui, H., Hamamoto, H., Sekimizu, K., Iwatsuki, M., Kim, Y. P., and Tomoda, H. (2014) In vitro and in vivo anti-MRSA activities of nosokomycins. Drug Discov. Ther. 8, 249-254
  82. Tsubota, T., Uchino, K., Kamimura, M., Ishikawa, M., Hamamoto, H., Sekimizu, K., and Sezutsu, H. (2014) Establishment of transgenic silkworms expressing GAL4 specifically in the haemocyte oenocytoid cells. Insect Mol. Biol. 23, 165-174
  83. Omae, Y., Sekimizu, K., and Kaito, C. (2014) Identification of Staphylococcus aureus colony-spreading stimulatory factors from mammalian serum. PLoS One 9, e97670
  84. Numata, S., Nagata, M., Mao, H., Sekimizu, K., and Kaito, C. (2014) CvfA Protein and Polynucleotide Phosphorylase Act in an Opposing Manner to Regulate Staphylococcus aureus Virulence. J. Biol. Chem. 289, 8420-8431
  85. Miyashita, A., Kizaki, H., Kawasaki, K., Sekimizu, K., and Kaito, C. (2014) Primed immune responses to gram-negative peptidoglycans confer infection resistance in silkworms. J. Biol. Chem. 289, 14412-14421
  86. Matsumoto, Y., Ishii, M., Ishii, K., Miyaguchi, W., Horie, R., Inagaki, Y., Hamamoto, H., Tatematsu, K., Uchino, K., Tamura, T., Sezutsu, H., and Sekimizu, K. (2014) Transgenic silkworms expressing human insulin receptors for evaluation of therapeutically active insulin receptor agonists. Biochem. Biophys. Res. Commun. 455, 159-164
  87. Kaito, C., and Sekimizu, K. (2014) Western Blotting for Staphylococcus aureus AgrA. Bio-protocol 4, e1071
  88. Ishii, K., Hamamoto, H., and Sekimizu, K. (2014) Establishment of a bacterial infection model using the European honeybee, Apis mellifera L. PLoS One 9, e89917
  89. Ishii, K., Adachi, T., Yasukawa, J., Suzuki, Y., Hamamoto, H., and Sekimizu, K. (2014) Induction of Virulence Gene Expression in Staphylococcus aureus by Pulmonary Surfactant. Infect. Immun. 82, 1500-1510
  90. Ishii, K., Adachi, T., Hara, T., Hamamoto, H., and Sekimizu, K. (2014) Identification of a Serratia marcescens virulence factor that promotes hemolymph bleeding in the silkworm, Bombyx mori. J. Invertebr. Pathol. 117, 61-67
  91. Ishii, K., Adachi, T., Hamamoto, H., and Sekimizu, K. (2014) Serratia marcescens suppresses host cellular immunity via the production of an adhesion-inhibitory factor against immunosurveillance cells. J. Biol. Chem. 289, 5876-5888
  92. Imamura, K., Imamachi, N., Akizuki, G., Kumakura, M., Kawaguchi, A., Nagata, K., Kato, A., Kawaguchi, Y., Sato, H., Yoneda, M., Kai, C., Yada, T., Suzuki, Y., Yamada, T., Ozawa, T., Kaneki, K., Inoue, T., Kobayashi, M., Kodama, T., Wada, Y., Sekimizu, K., and Akimitsu, N. (2014) Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Mol. Cell 53, 393-406
  93. Ikuo, M., Nagano, G., Saito, Y., Mao, H., Sekimizu, K., and Kaito, C. (2014) Inhibition of exotoxin production by mobile genetic element SCCmec-encoded psm-mec RNA is conserved in staphylococcal species. PLoS One 9, e100260
  94. Aoyagi, T., Kaito, C., Sekimizu, K., Omae, Y., Saito, Y., Mao, H., Inomata, S., Hatta, M., Endo, S., Kanamori, H., Gu, Y., Tokuda, K., Yano, H., Kitagawa, M., and Kaku, M. (2014) Impact of psm-mec in the mobile genetic element on the clinical characteristics and outcome of SCCmec-II methicillin-resistant Staphylococcus aureus bacteraemia in Japan. Clin. Microbiol. Infect. 20, 912-919
  95. Adachi, T., Ishii, K., Matsumoto, Y., Hayashi, Y., Hamamoto, H., and Sekimizu, K. (2014) Niemann-Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines. Biochem. J. 459, 137-147
  96. Paudel, A., Kaneko, K., Watanabe, A., Matsunaga, S., Kanai, M., Hamamoto, H., and Sekimizu, K. (2013) Structure-activity relationship study of novel iminothiadiazolo-pyrimidinone antimicrobial agents. J. Antibiot. (Tokyo) 66, 663-667
  97. Omae, Y., Hanada, Y., Sekimizu, K., and Kaito, C. (2013) Silkworm apolipophorin protein inhibits hemolysin gene expression of Staphylococcus aureus via binding to cell surface lipoteichoic acids. J. Biol. Chem. 288, 25542-25550
  98. Kaito, C., Saito, Y., Ikuo, M., Omae, Y., Mao, H., Nagano, G., Fujiyuki, T., Numata, S., Han, X., Obata, K., Hasegawa, S., Yamaguchi, H., Inokuchi, K., Ito, T., Hiramatsu, K., and Sekimizu, K. (2013) Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. PLoS Pathog. 9, e1003269
  99. Ishii, K., Adachi, T., Hamamoto, H., Oonishi, T., Kamimura, M., Imamura, K., and Sekimizu, K. (2013) Insect cytokine paralytic peptide activates innate immunity via nitric oxide production in the silkworm Bombyx mori. Dev. Comp. Immunol. 39, 147-153
  100. Hu, Y., Hamamoto, H., and Sekimizu, K. (2013) Use of silkworms to evaluate the pathogenicity of bacteria attached to cedar pollen. Drug Discov. Ther. 7, 153-157
  101. Dzoyem, J. P., Hamamoto, H., Ngameni, B., Ngadjui, B. T., and Sekimizu, K. (2013) Antimicrobial action mechanism of flavonoids from Dorstenia species. Drug Discov. Ther. 7, 66-72
  102. Anantaworasakul, P., Hamamoto, H., Sekimizu, K., and Okonogi, S. (2013) Physiological comparison between mulberry (‘Morus alba’L.) leaves diet and artificial diet on growth development as well as antibiotic therapeutic response of silkworms. Australian Journal of Crop Science 7, 2029
  103. Shiratsuchi, A., Mori, T., Sakurai, K., Nagaosa, K., Sekimizu, K., Lee, B. L., and Nakanishi, Y. (2012) Independent recognition of Staphylococcus aureus by two receptors for phagocytosis in Drosophila. J. Biol. Chem. 287, 21663-21672
  104. Paudel, A., Hamamoto, H., Kobayashi, Y., Yokoshima, S., Fukuyama, T., and Sekimizu, K. (2012) Identification of novel deoxyribofuranosyl indole antimicrobial agents. J. Antibiot. (Tokyo) 65, 53-57
  105. Omae, Y., Sekimizu, K., and Kaito, C. (2012) Inhibition of colony-spreading activity of Staphylococcus aureus by secretion of delta-hemolysin. J. Biol. Chem. 287, 15570-15579
  106. Nakayama, M., Kurokawa, K., Nakamura, K., Lee, B. L., Sekimizu, K., Kubagawa, H., Hiramatsu, K., Yagita, H., Okumura, K., Takai, T., Underhill, D. M., Aderem, A., and Ogasawara, K. (2012) Inhibitory Receptor Paired Ig-like Receptor B Is Exploited by Staphylococcus aureus for Virulence. J. Immunol. 189, 5903-5911
  107. Miyazaki, S., Matsumoto, Y., Sekimizu, K., and Kaito, C. (2012) Evaluation of Staphylococcus aureus virulence factors using a silkworm model. FEMS Microbiol. Lett. 326, 116-124
  108. Miyashita, A., Iyoda, S., Ishii, K., Hamamoto, H., Sekimizu, K., and Kaito, C. (2012) Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals. FEMS Microbiol. Lett. 333, 59-68
  109. Matsumoto, Y., Miyazaki, S., Fukunaga, D. H., Shimizu, K., Kawamoto, S., and Sekimizu, K. (2012) Quantitative evaluation of cryptococcal pathogenesis and antifungal drugs using a silkworm infection model with Cryptococcus neoformans. J. Appl. Microbiol. 112, 138-146
  110. Kushida, A., Horie, R., Hattori, K., Hamamoto, H., Sekimizu, K., and Tamura, H. (2012) Xanthurenic acid is an endogenous substrate for the silkworm cytosolic sulfotransferase, bmST1. J. Insect Physiol. 58, 83-88
  111. Ishii, K., Adachi, T., Imamura, K., Takano, S., Usui, K., Suzuki, K., Hamamoto, H., Watanabe, T., and Sekimizu, K. (2012) Serratia marcescens Induces Apoptotic Cell Death in Host Immune Cells via a Lipopolysaccharide- and Flagella-dependent Mechanism. J. Biol. Chem. 287, 36582-36592
  112. Inagaki, Y., Matsumoto, Y., Kataoka, K., Matsuhashi, N., and Sekimizu, K. (2012) Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in silkworm hemolymph. BMC Pharmacol Toxicol 13, 13
  113. Fujiyuki, T., Hamamoto, H., Ishii, K., Urai, M., Kataoka, K., Takeda, T., Shibata, S., and Sekimizu, K. (2012) Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay. Drug Discov. Ther. 6, 88-93
  114. Ueno, K., Matsumoto, Y., Uno, J., Sasamoto, K., Sekimizu, K., Kinjo, Y., and Chibana, H. (2011) Intestinal Resident Yeast Candida glabrata Requires Cyb2p-Mediated Lactate Assimilation to Adapt in Mouse Intestine. PLoS One 6, e24759
  115. Ueda, T., Kaito, C., Omae, Y., and Sekimizu, K. (2011) Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus. Microb. Pathog. 51, 178-185
  116. Morishita, D., Takami, M., Yoshikawa, S., Katayama, R., Sato, S., Kukimoto-Niino, M., Umehara, T., Shirouzu, M., Sekimizu, K., Yokoyama, S., and Fujita, N. (2011) Cell-permeable carboxyl-terminal p27(Kip1) peptide exhibits anti-tumor activity by inhibiting Pim-1 kinase. J. Biol. Chem. 286, 2681-2688
  117. Matsumoto, Y., Sumiya, E., Sugita, T., and Sekimizu, K. (2011) An invertebrate hyperglycemic model for the identification of anti-diabetic drugs. PLoS One 6, e18292
  118. Kaito, C., Usui, K., Kyuma, T., and Sekimizu, K. (2011) Isolation of mammalian pathogenic bacteria using silkworms. Drug Discov. Ther. 5, 66-70
  119. Kaito, C., Saito, Y., Nagano, G., Ikuo, M., Omae, Y., Hanada, Y., Han, X., Kuwahara-Arai, K., Hishinuma, T., Baba, T., Ito, T., Hiramatsu, K., and Sekimizu, K. (2011) Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. PLoS Pathog. 7, e1001267
  120. Kaito, C., Hirano, T., Omae, Y., and Sekimizu, K. (2011) Digestion of extracellular DNA is required for giant colony formation of Staphylococcus aureus. Microb. Pathog. 51, 142-148
  121. Imamura, K., Ishii, K., Hamamoto, H., and Sekimizu, K. (2011) Hemocytes and humoral factors in silkworm blood are cooperatively involved in sheep erythrocyte aggregation. Drug Discov. Ther. 5, 125-129
  122. Hanada, Y., Sekimizu, K., and Kaito, C. (2011) Silkworm apolipophorin protein inhibits Staphylococcus aureus virulence. J. Biol. Chem. 286, 39360-39369
  123. Fukuyama, N., Ino, C., Suzuki, Y., Kobayashi, N., Hamamoto, H., Sekimizu, K., and Orihara, Y. (2011) Antimicrobial sesquiterpenoids from Laurus nobilis L. Nat Prod Res 25, 1295-1303
  124. Dhital, S., Hamamoto, H., Urai, M., Ishii, K., and Sekimizu, K. (2011) Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay. Drug Discov. Ther. 5, 18-25
  125. Arimitsu, N., Kogure, T., Baba, T., Nakao, K., Hamamoto, H., Sekimizu, K., Yamamoto, A., Nakanishi, H., Taguchi, R., Tagaya, M., and Tani, K. (2011) p125/Sec23-interacting protein (Sec23ip) is required for spermiogenesis. FEBS Lett. 585, 2171-2176
  126. Wang, D. L., Qi, F. H., Xu, H. L., Inagaki, Y., Orihara, Y., Sekimizu, K., Kokudo, N., Wang, F. S., and Tang, W. (2010) Apoptosis-inducing activity of compounds screened and characterized from cinobufacini by bioassay-guided isolation. Mol Med Rep 3, 717-722
  127. Tano, K., Hamamoto, H., Ito, T., Sumiya, E., Rakwal, R., Shibato, J., Masuo, Y., Ijiri, K., Sekimizu, K., and Akimitsu, N. (2010) Reduced expression of Sytl 1 and Ccdc21 and impaired induction of Mt I by oxidative stress in SII-K1 knockout mice. Drug Discov. Ther. 4, 368-372
  128. Tabuchi, Y., Shiratsuchi, A., Kurokawa, K., Gong, J. H., Sekimizu, K., Lee, B. L., and Nakanishi, Y. (2010) Inhibitory role for D-alanylation of wall teichoic acid in activation of insect Toll pathway by peptidoglycan of Staphylococcus aureus. J. Immunol. 185, 2424-2431
  129. Shiratsuchi, A., Shimizu, K., Watanabe, I., Hashimoto, Y., Kurokawa, K., Razanajatovo, I. M., Park, K. H., Park, H. K., Lee, B. L., Sekimizu, K., and Nakanishi, Y. (2010) Auxiliary role for D-alanylated wall teichoic acid in Toll-like receptor 2-mediated survival of Staphylococcus aureus in macrophages. Immunology 129, 268-277
  130. Matsuo, M., Kurokawa, K., Lee, B. L., and Sekimizu, K. (2010) Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus. Biol. Pharm. Bull. 33, 198-203
  131. Matsumoto, Y., Xu, Q., Miyazaki, S., Kaito, C., Farr, C. L., Axelrod, H. L., Chiu, H. J., Klock, H. E., Knuth, M. W., Miller, M. D., Elsliger, M. A., Deacon, A. M., Godzik, A., Lesley, S. A., Sekimizu, K., and Wilson, I. A. (2010) Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module. Structure 18, 537-547
  132. Koyama, H., Ueda, T., Ito, T., and Sekimizu, K. (2010) Novel RNA polymerase II mutation suppresses transcriptional fidelity and oxidative stress sensitivity in rpb9Delta yeast. Genes Cells 15, 151-159
  133. Ishii, K., Hamamoto, H., Kamimura, M., Nakamura, Y., Noda, H., Imamura, K., Mita, K., and Sekimizu, K. (2010) Insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm Bombyx mori. J. Biol. Chem. 285, 28635-28642
  134. Ishii, K., Hamamoto, H., Imamura, K., Adachi, T., Shoji, M., Nakayama, K., and Sekimizu, K. (2010) Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. J. Biol. Chem. 285, 33338-33347
  135. Ikuo, M., Kaito, C., and Sekimizu, K. (2010) The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene. Microb. Pathog. 49, 1-7
  136. Fujiyuki, T., Imamura, K., Hamamoto, H., and Sekimizu, K. (2010) Evaluation of therapeutic effects and pharmacokinetics of antibacterial chromogenic agents in a silkworm model of Staphylococcus aureus infection. Drug Discov. Ther. 4, 349-354
  137. Asami, Y., Horie, R., Hamamoto, H., and Sekimizu, K. (2010) Use of silkworms for identification of drug candidates having appropriate pharmacokinetics from plant sources. BMC Pharmacol. 10, 7
  138. Usui, K., Miyazaki, S., Kaito, C., and Sekimizu, K. (2009) Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity. Microb. Pathog. 46, 59-62
  139. Takabatake, N., Iseki, H., Ikehara, Y., Kanuka, H., Yokoyama, N., Sekimizu, K., and Igarashi, I. (2009) Isolation and pathogenic characterization of an OB1 variant of Babesia rodhaini which has a glycophorin A-independent pathway to murine red blood cells. Vet. Parasitol. 159, 97-104
  140. Okuyama-Nishida, Y., Akiyama, N., Sugimori, G., Nomura, K., Ogawa, K., Homma, K. J., Sekimizu, K., Tsujimoto, M., and Natori, S. (2009) Prevention of death in bacterium-infected mice by a synthetic antimicrobial peptide, L5, through activation of host immunity. Antimicrob. Agents Chemother. 53, 2510-2516
  141. Oku, Y., Kurokawa, K., Matsuo, M., Yamada, S., Lee, B. L., and Sekimizu, K. (2009) Pleiotropic roles of polyglycerolphosphate synthase of lipoteichoic acid in growth of Staphylococcus aureus cells. J. Bacteriol. 191, 141-151
  142. Nagata, M., Ito, T., Arimitsu, N., Koyama, H., and Sekimizu, K. (2009) Transcription arrest relief by S-II/TFIIS during gene expression in erythroblast differentiation. Genes Cells 14, 371-380
  143. Kurokawa, K., Mizumura, H., Takaki, T., Ishii, Y., Ichihashi, N., Lee, B. L., and Sekimizu, K. (2009) Rapid exchange of bound ADP on the Staphylococcus aureus replication initiation protein DnaA. J. Biol. Chem. 284, 34201-34210
  144. Kurokawa, K., Lee, H., Roh, K. B., Asanuma, M., Kim, Y. S., Nakayama, H., Shiratsuchi, A., Choi, Y., Takeuchi, O., Kang, H. J., Dohmae, N., Nakanishi, Y., Akira, S., Sekimizu, K., and Lee, B. L. (2009) The Triacylated ATP Binding Cluster Transporter Substrate-binding Lipoprotein of Staphylococcus aureus Functions as a Native Ligand for Toll-like Receptor 2. J. Biol. Chem. 284, 8406-8411
  145. Kurokawa, K., Hamamoto, H., Matsuo, M., Nishida, S., Yamane, N., Lee, B. L., Murakami, K., Maki, H., and Sekimizu, K. (2009) Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model. Antimicrob. Agents Chemother. 53, 4025-4027
  146. Hashimoto, Y., Tabuchi, Y., Sakurai, K., Kutsuna, M., Kurokawa, K., Awasaki, T., Sekimizu, K., Nakanishi, Y., and Shiratsuchi, A. (2009) Identification of lipoteichoic acid as a ligand for draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes. J. Immunol. 183, 7451-7460
  147. Hamamoto, H., Tonoike, A., Narushima, K., Horie, R., and Sekimizu, K. (2009) Silkworm as a model animal to evaluate drug candidate toxicity and metabolism. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 149, 334-339
  148. Fujiyuki, T., Matsuzaka, E., Nakaoka, T., Takeuchi, H., Wakamoto, A., Ohka, S., Sekimizu, K., Nomoto, A., and Kubo, T. (2009) Distribution of Kakugo virus and its effects on the gene expression profile in the brain of the worker honeybee Apis mellifera L. J. Virol. 83, 11560-11568
  149. Shiokawa, K., Aso, M., Kondo, T., Uchiyama, H., Kuroyanagi, S., Takai, J., Takahashi, S., Kajitani, M., Kaito, C., Sekimizu, K., Takayama, E., Igarashi, K., and Hara, H. (2008) Gene expression in Pre-MBT embryos and activation of maternally-inherited program of apoptosis to be executed at around MBT as a fail-safe mechanism in Xenopus early embryogenesis. Gene Regul. Syst. Bio. 2, 213-231
  150. Orihara, Y., Hamamoto, H., Kasuga, H., Shimada, T., Kawaguchi, Y., and Sekimizu, K. (2008) A silkworm baculovirus model for assessing the therapeutic effects of antiviral compounds: characterization and application to the isolation of antivirals from traditional medicines. J. Gen. Virol. 89, 188-194
  151. Nagata, M., Kaito, C., and Sekimizu, K. (2008) Phosphodiesterase activity of CvfA is required for virulence in Staphylococcus aureus. J. Biol. Chem. 283, 2176-2184
  152. Morishita, D., Katayama, R., Sekimizu, K., Tsuruo, T., and Fujita, N. (2008) Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels. Cancer Res. 68, 5076-5085
  153. Kurokawa, K., Nishida, S., Ishibashi, M., Mizumura, H., Ueno, K., Yutsudo, T., Maki, H., Murakami, K., and Sekimizu, K. (2008) Staphylococcus aureus MurC participates in L-alanine recognition via histidine 343, a conserved motif in the shallow hydrophobic pocket. J. Biochem. 143, 417-424
  154. Koyama, H., Sumiya, E., Nagata, M., Ito, T., and Sekimizu, K. (2008) Transcriptional repression of the IMD2 gene mediated by the transcriptional co-activator Sub1. Genes Cells 13, 1113-1126
  155. Koyama, H., Sumiya, E., Ito, T., and Sekimizu, K. (2008) Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae. FEMS Yeast Res 8, 193-194
  156. Kaito, C., Omae, Y., Matsumoto, Y., Nagata, M., Yamaguchi, H., Aoto, T., Ito, T., Hiramatsu, K., and Sekimizu, K. (2008) A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. PLoS One 3, e3921
  157. Ishii, K., Hamamoto, H., Sasaki, T., Ikegaya, Y., Yamatsugu, K., Kanai, M., Shibasaki, M., and Sekimizu, K. (2008) Pharmacologic action of oseltamivir on the nervous system. Drug Discov. Ther. 2, 24-34
  158. Ishii, K., Hamamoto, H., Kamimura, M., and Sekimizu, K. (2008) Activation of the silkworm cytokine by bacterial and fungal cell wall components via a reactive oxygen species-triggered mechanism. J. Biol. Chem. 283, 2185-2191
  159. Iijima, R., Kunieda, T., Yamaguchi, S., Kamigaki, H., Fujii-Taira, I., Sekimizu, K., Kubo, T., Natori, S., and Homma, K. J. (2008) The extracellular adenosine deaminase growth factor, ADGF/CECR1, plays a role in Xenopus embryogenesis via the adenosine/P1 receptor. J. Biol. Chem. 283, 2255-2264
  160. Takabatake, N., Okamura, M., Yokoyama, N., Ikehara, Y., Akimitsu, N., Arimitsu, N., Hamamoto, H., Sekimizu, K., Suzuki, H., and Igarashi, I. (2007) Glycophorin A-knockout mice, which lost sialoglycoproteins from the red blood cell membrane, are resistant to lethal infection of Babesia rodhaini. Vet. Parasitol. 148, 93-101
  161. Nagata, M., Arimitsu, N., Ito, T., and Sekimizu, K. (2007) Antioxidant N-acetyl-L-cysteine inhibits erythropoietin-induced differentiation of erythroid progenitors derived from mouse fetal liver. Cell Biol. Int. 31, 252-256
  162. Matsumoto, Y., Kaito, C., Morishita, D., Kurokawa, K., and Sekimizu, K. (2007) Regulation of exoprotein gene expression by the Staphylococcus aureus cvfB gene. Infect. Immun. 75, 1964-1972
  163. Li, Y., Kurokawa, K., Reutimann, L., Mizumura, H., Matsuo, M., and Sekimizu, K. (2007) dnaB and dnaI temperature-sensitive mutants of Staphylococcus aureus: evidence for involvement of DnaB and DnaI in synchrony regulation of chromosome replication. Microbiology 153, 3370-3379
  164. Kurokawa, K., Kaito, C., and Sekimizu, K. (2007) Two-component signaling in the virulence of Staphylococcus aureus: a silkworm larvae-pathogenic agent infection model of virulence. Methods Enzymol. 422, 233-244
  165. Koyama, H., Ito, T., Nakanishi, T., and Sekimizu, K. (2007) Stimulation of RNA polymerase II transcript cleavage activity contributes to maintain transcriptional fidelity in yeast. Genes Cells 12, 547-559
  166. Kaito, C., and Sekimizu, K. (2007) Colony spreading in Staphylococcus aureus. J. Bacteriol. 189, 2553-2557
  167. Ito, T., Saso, K., Arimitsu, N., and Sekimizu, K. (2007) Defective FESTA/EAF2-mediated transcriptional activation in S-II-deficient embryonic stem cells. Biochem. Biophys. Res. Commun. 363, 603-609
  168. Ito, T., Doi, K., Matsumoto, N., Kakihara, F., Noiri, E., Hasegawa, S., Tokunaga, K., and Sekimizu, K. (2007) Lack of polymorphisms in the coding region of the highly conserved gene encoding transcription elongationfactor S-II (TCEA1). Drug Discov. Ther. 1, 9-11
  169. Ishino, T., Kunieda, T., Natori, S., Sekimizu, K., and Kubo, T. (2007) Identification of novel members of the Xenopus Ca2+ -dependent lectin family and analysis of their gene expression during tail regeneration and development. J. Biochem. 141, 479-488
  170. Ishii, K., Hamamoto, H., and Sekimizu, K. (2007) A novel method to suppress the dispersal of Japanese cedar pollen by inducing morphologic changes with weak alkaline solutions. Drug Discov. Ther. 1, 124-129
  171. Ishibashi, M., Kurokawa, K., Nishida, S., Ueno, K., Matsuo, M., and Sekimizu, K. (2007) Isolation of temperature-sensitive mutations in murC of Staphylococcus aureus. FEMS Microbiol. Lett. 274, 204-209
  172. Ikeda, R., Saito, F., Matsuo, M., Kurokawa, K., Sekimizu, K., Yamaguchi, M., and Kawamoto, S. (2007) Contribution of the mannan backbone of cryptococcal glucuronoxylomannan and a glycolytic enzyme of Staphylococcus aureus to contact-mediated killing of Cryptococcus neoformans. J. Bacteriol. 189, 4815-4826
  173. Hattori, K., Hirayama, M., Suzuki, H., Hamamoto, H., Sekimizu, K., and Tamura, H. O. (2007) Cloning and expression of a novel sulfotransferase with unique substrate specificity from Bombyx mori. Biosci. Biotechnol. Biochem. 71, 1044-1051
  174. Ashibe, B., Hirai, T., Higashi, K., Sekimizu, K., and Motojima, K. (2007) Dual subcellular localization in the endoplasmic reticulum and peroxisomes and a vital role in protecting against oxidative stress of fatty aldehyde dehydrogenase are achieved by alternative splicing. J. Biol. Chem. 282, 20763-20773
  175. Nishida, S., Kurokawa, K., Matsuo, M., Sakamoto, K., Ueno, K., Kita, K., and Sekimizu, K. (2006) Identification and characterization of amino acid residues essential for the active site of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus. J. Biol. Chem. 281, 1714-1724
  176. Murai, N., Kurokawa, K., Ichihashi, N., Matsuo, M., and Sekimizu, K. (2006) Isolation of a temperature-sensitive dnaA mutant of Staphylococcus aureus. FEMS Microbiol. Lett. 254, 19-26
  177. Kaito, C., Morishita, D., Matsumoto, Y., Kurokawa, K., and Sekimizu, K. (2006) Novel DNA binding protein SarZ contributes to virulence in Staphylococcus aureus. Mol. Microbiol. 62, 1601-1617
  178. Ito, T., Arimitsu, N., Takeuchi, M., Kawamura, N., Nagata, M., Saso, K., Akimitsu, N., Hamamoto, H., Natori, S., Miyajima, A., and Sekimizu, K. (2006) Transcription elongation factor S-II is required for definitive hematopoiesis. Mol. Cell. Biol. 26, 3194-3203
  179. Hossain, M. S., Hamamoto, H., Matsumoto, Y., Razanajatovo, I. M., Larranaga, J., Kaito, C., Kasuga, H., and Sekimizu, K. (2006) Use of silkworm larvae to study pathogenic bacterial toxins. J. Biochem. 140, 439-444
  180. Eguchi, Y., Ueno, S., Kaito, C., Sekimizu, K., and Shiokawa, K. (2006) Cleavage and survival of Xenopus embryos exposed to 8 T static magnetic fields in a rotating clinostat. Bioelectromagnetics 27, 307-313
  181. Yamaguchi, S., Katagiri, S., Sekimizu, K., Natori, S., and Homma, K. J. (2005) Involvement of EDTP, an egg-derived tyrosine phosphatase, in the early development of Drosophila melanogaster. J. Biochem. 138, 721-728
  182. Shiokawa, K., Takayama, E., Higo, T., Kuroyanagi, S., Kaito, C., Hara, H., Kajitani, M., Sekimizu, K., Tadakuma, T., Miura, K., Igarashi, K., and Yaoita, Y. (2005) Occurrence of pre-MBT synthesis of caspase-8 mRNA and activation of caspase-8 prior to execution of SAMDC (S-adenosylmethionine decarboxylase)-induced, but not p53-induced, apoptosis in Xenopus late blastulae. Biochem. Biophys. Res. Commun. 336, 682-691
  183. Sekimizu, K., Larranaga, J., Hamamoto, H., Sekine, M., Furuchi, T., Katane, M., Homma, H., and Matsuki, N. (2005) D-Glutamic acid-induced muscle contraction in the silkworm, Bombyx mori. J. Biochem. 137, 199-203
  184. Rungruang, T., Kaneko, O., Murakami, Y., Tsuboi, T., Hamamoto, H., Akimitsu, N., Sekimizu, K., Kinoshita, T., and Torii, M. (2005) Erythrocyte surface glycosylphosphatidyl inositol anchored receptor for the malaria parasite. Mol. Biochem. Parasitol. 140, 13-21
  185. Kawamura, N., Kurokawa, K., Ito, T., Hamamoto, H., Koyama, H., Kaito, C., and Sekimizu, K. (2005) Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation. Genes Cells 10, 477-487
  186. Kaito, C., Kurokawa, K., Matsumoto, Y., Terao, Y., Kawabata, S., Hamada, S., and Sekimizu, K. (2005) Silkworm pathogenic bacteria infection model for identification of novel virulence genes. Mol. Microbiol. 56, 934-944
  187. Hossain, M. S., Kurokawa, K., and Sekimizu, K. (2005) Induction of fusion-competent myoblast-specific gene expression during myogenic differentiation of Drosophila Schneider cells by DNA double-strand breaks or replication inhibition. Biochim. Biophys. Acta 1743, 176-186
  188. Hamamoto, H., Kamura, K., Razanajatovo, I. M., Murakami, K., Santa, T., and Sekimizu, K. (2005) Effects of molecular mass and hydrophobicity on transport rates through non-specific pathways of the silkworm larva midgut. Int. J. Antimicrob. Agents 26, 38-42
  189. Aramaki, Y., Ogawa, K., Toh, Y., Ito, T., Akimitsu, N., Hamamoto, H., Sekimizu, K., Matsusue, K., Kono, A., Iguchi, H., and Takiguchi, S. (2005) Direct interaction between metastasis-associated protein 1 and endophilin 3. FEBS Lett. 579, 3731-3736
  190. Takeuchi, K., Takahashi, H., Sugai, M., Iwai, H., Kohno, T., Sekimizu, K., Natori, S., and Shimada, I. (2004) Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR. J. Biol. Chem. 279, 4981-4987
  191. Okuyama, Y., Cho, J. H., Nakajima, Y., Homma, K., Sekimizu, K., and Natori, S. (2004) Binding between azurocidin and calreticulin: its involvement in the activation of peripheral monocytes. J. Biochem. 135, 171-177
  192. Oku, Y., Kurokawa, K., Ichihashi, N., and Sekimizu, K. (2004) Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol. Microbiology 150, 45-51
  193. Nakata, A., Ito, T., Nagata, M., Hori, S., and Sekimizu, K. (2004) GRIP1tau, a novel PDZ domain-containing transcriptional activator, cooperates with the testis-specific transcription elongation factor SII-T1. Genes Cells 9, 1125-1135
  194. Li, Y., Kurokawa, K., Matsuo, M., Fukuhara, N., Murakami, K., and Sekimizu, K. (2004) Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replication. Mol. Genet. Genomics 271, 447-457
  195. Jilma, B., Akimitsu, N., Fukuma, N., Sekimizu, K., and Jilma-Stohlawetz, P. (2004) Man, mouse and Duffy genotype-phenotype-specific pharmacokinetics of monocyte chemotactic protein-1. Transfus. Med. 14, 251-252
  196. Ishida, T., Akimitsu, N., Kashioka, T., Hatano, M., Kubota, T., Ogata, Y., Sekimizu, K., and Katayama, T. (2004) DiaA, a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication. J. Biol. Chem. 279, 45546-45555
  197. Hossain, M. S., Kurokawa, K., Akimitsu, N., and Sekimizu, K. (2004) DNA topoisomerase II is required for the G0-to-S phase transition in Drosophila Schneider cells, but not in yeast. Genes Cells 9, 905-917
  198. Hamamoto, H., Kurokawa, K., Kaito, C., Kamura, K., Manitra Razanajatovo, I., Kusuhara, H., Santa, T., and Sekimizu, K. (2004) Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. Antimicrob. Agents Chemother. 48, 774-779
  199. Akimitsu, N., Kim, H. S., Hamamoto, H., Kamura, K., Fukuma, N., Arimitsu, N., Ono, K., Wataya, Y., Torii, M., and Sekimizu, K. (2004) Duffy antigen is important for the lethal effect of the lethal strain of Plasmodium yoelii 17XL. Parasitol. Res. 93, 499-503
  200. Ubukata, T., Shimizu, T., Adachi, N., Sekimizu, K., and Nakanishi, T. (2003) Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II. J. Biol. Chem. 278, 8580-8585
  201. Saso, K., Ito, T., Natori, S., and Sekimizu, K. (2003) Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II. J. Biochem. 133, 493-500
  202. Matsuo, M., Kurokawa, K., Nishida, S., Li, Y., Takimura, H., Kaito, C., Fukuhara, N., Maki, H., Miura, K., Murakami, K., and Sekimizu, K. (2003) Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus. FEMS Microbiol. Lett. 222, 107-113
  203. Koyama, H., Ito, T., Nakanishi, T., Kawamura, N., and Sekimizu, K. (2003) Transcription elongation factor S-II maintains transcriptional fidelity and confers oxidative stress resistance. Genes Cells 8, 779-788
  204. Kashiwazaki, M., Tanaka, T., Kanda, H., Ebisuno, Y., Izawa, D., Fukuma, N., Akimitsu, N., Sekimizu, K., Monden, M., and Miyasaka, M. (2003) A high endothelial venule-expressing promiscuous chemokine receptor DARC can bind inflammatory, but not lymphoid, chemokines and is dispensable for lymphocyte homing under physiological conditions. Int. Immunol. 15, 1219-1227
  205. Ishino, T., Shirai, M., Kunieda, T., Sekimizu, K., Natori, S., and Kubo, T. (2003) Identification of genes induced in regenerating Xenopus tadpole tails by using the differential display method. Dev. Dyn. 226, 317-325
  206. Ichihashi, N., Kurokawa, K., Matsuo, M., Kaito, C., and Sekimizu, K. (2003) Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication. J. Biol. Chem. 278, 28778-28786
  207. Hossain, M. S., Akimitsu, N., Kurokawa, K., and Sekimizu, K. (2003) Myogenic differentiation of Drosophila Schneider cells by DNA double-strand break-inducing drugs. Differentiation 71, 271-280
  208. Fukuma, N., Akimitsu, N., Hamamoto, H., Kusuhara, H., Sugiyama, Y., and Sekimizu, K. (2003) A role of the Duffy antigen for the maintenance of plasma chemokine concentrations. Biochem. Biophys. Res. Commun. 303, 137-139
  209. Arimitsu, N., Akimitsu, N., Kotani, N., Takasaki, S., Kina, T., Hamamoto, H., Kamura, K., and Sekimizu, K. (2003) Glycophorin A requirement for expression of O-linked antigens on the erythrocyte membrane. Genes Cells 8, 769-777
  210. Akimitsu, N., Kamura, K., Tone, S., Sakaguchi, A., Kikuchi, A., Hamamoto, H., and Sekimizu, K. (2003) Induction of apoptosis by depletion of DNA topoisomerase IIalpha in mammalian cells. Biochem. Biophys. Res. Commun. 307, 301-307
  211. Akimitsu, N., Adachi, N., Hirai, H., Hossain, M. S., Hamamoto, H., Kobayashi, M., Aratani, Y., Koyama, H., and Sekimizu, K. (2003) Enforced cytokinesis without complete nuclear division in embryonic cells depleting the activity of DNA topoisomerase IIalpha. Genes Cells 8, 393-402
  212. Nishida, S., Fujimitsu, K., Sekimizu, K., Ohmura, T., Ueda, T., and Katayama, T. (2002) A nucleotide switch in the Escherichia coli DnaA protein initiates chromosomal replication: evidnece from a mutant DnaA protein defective in regulatory ATP hydrolysis in vitro and in vivo. J. Biol. Chem. 277, 14986-14995
  213. Nakanishi, T., and Sekimizu, K. (2002) SDT1/SSM1, a multicopy suppressor of S-II null mutant, encodes a novel pyrimidine 5′-nucleotidase. J. Biol. Chem. 277, 22103-22106
  214. Koyanagi-Katsuta, R., Akimitsu, N., Hamamoto, H., Arimitsu, N., Hatano, T., and Sekimizu, K. (2002) Embryonic lethality of mutant mice deficient in the p116 gene. J. Biochem. 131, 833-837
  215. Kaito, C., Kurokawa, K., Hossain, M. S., Akimitsu, N., and Sekimizu, K. (2002) Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene. FEMS Microbiol. Lett. 210, 157-164
  216. Kaito, C., Akimitsu, N., Watanabe, H., and Sekimizu, K. (2002) Silkworm larvae as an animal model of bacterial infection pathogenic to humans. Microb. Pathog. 32, 183-190
  217. Hossain, M. S., Akimitsu, N., Takaki, T., Hirai, H., and Sekimizu, K. (2002) ICRF-193, a catalytic inhibitor of DNA topoisomerase II, inhibits re-entry into the cell division cycle from quiescent state in mammalian cells. Genes Cells 7, 285-294
  218. Aozasa, N., Kubo, T., Sekimizu, K., and Natori, S. (2002) Identification and characterization of a Drosophila homologue of ATBP. Insect Mol. Biol. 11, 31-36
  219. Takeuchi, H., Kage, E., Sawata, M., Kamikouchi, A., Ohashi, K., Ohara, M., Fujiyuki, T., Kunieda, T., Sekimizu, K., Natori, S., and Kubo, T. (2001) Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honeybee brain. Insect Mol. Biol. 10, 487-494
  220. Kuroda, M., Ohta, T., Uchiyama, I., Baba, T., Yuzawa, H., Kobayashi, I., Cui, L., Oguchi, A., Aoki, K., Nagai, Y., Lian, J., Ito, T., Kanamori, M., Matsumaru, H., Maruyama, A., Murakami, H., Hosoyama, A., Mizutani-Ui, Y., Takahashi, N. K., Sawano, T., Inoue, R., Kaito, C., Sekimizu, K., Hirakawa, H., Kuhara, S., Goto, S., Yabuzaki, J., Kanehisa, M., Yamashita, A., Oshima, K., Furuya, K., Yoshino, C., Shiba, T., Hattori, M., Ogasawara, N., Hayashi, H., and Hiramatsu, K. (2001) Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet 357, 1225-1240
  221. Kubota, T., Ito, Y., Sekimizu, K., Tagaya, M., and Katayama, T. (2001) DnaA protein Lys-415 is close to the ATP-binding site: ATP-pyridoxal affinity labeling. Biochem. Biophys. Res. Commun. 288, 1141-1148
  222. Kawakami, H., Iwura, T., Takata, M., Sekimizu, K., Hiraga, S., and Katayama, T. (2001) Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA. Mol. Genet. Genomics 266, 167-179
  223. Kaito, C., Kai, M., Higo, T., Takayama, E., Fukamachi, H., Sekimizu, K., and Shiokawa, K. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2′-deoxycytidine and 5-methyl-2′-deoxycytidine-5′-triphosphate in Xenopus laevis embryos. Dev. Growth Differ. 43, 383-390
  224. Inoue, R., Kaito, C., Tanabe, M., Kamura, K., Akimitsu, N., and Sekimizu, K. (2001) Genetic identification of two distinct DNA polymerases, DnaE and PolC, that are essential for chromosomal DNA replication in Staphylococcus aureus. Mol. Genet. Genomics 266, 564-571
  225. Aozasa, N., Shiraishi, H., Nakanishi-Matsui, M., Kobayashi, A., Sekimizu, K., Kubo, T., and Natori, S. (2001) Activation of the Sarcophaga lectin gene promoter by (A + T)-stretch binding protein. Eur. J. Biochem. 268, 2506-2511
  226. Aozasa, N., Shiraishi, H., Kobayashi, A., Sekimizu, K., Kubo, T., and Natori, S. (2001) Interaction between ATBP and DmUbc9 in the expression of the Sarcophaga lectin gene. Biochem. Biophys. Res. Commun. 286, 949-952
  227. Takata, M., Guo, L., Katayama, T., Hase, M., Seyama, Y., Miki, T., and Sekimizu, K. (2000) Mutant DnaA proteins defective in duplex opening of oriC, the origin of chromosomal DNA replication in Escherichia coli. Mol. Microbiol. 35, 454-462
  228. Koyanagi-Katsuta, R., Akimitsu, N., Arimitsu, N., Hatano, T., and Sekimizu, K. (2000) Apoptosis of mouse embryonic stem cells induced by single cell suspension. Tissue Cell 32, 66-70
  229. Kondo, T., Mima, S., Fukuma, N., Sekimizu, K., Tsuchiya, T., and Mizushima, T. (2000) Suppression of temperature-sensitivity of a dnaA46 mutant by excessive DNA supercoiling. Biochem. J. 348 Pt 2, 375-379
  230. Kurokawa, K., Nishida, S., Emoto, A., Sekimizu, K., and Katayama, T. (1999) Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli. EMBO J. 18, 6642-6652
  231. Guo, L., Katayama, T., Seyama, Y., Sekimizu, K., and Miki, T. (1999) Isolation and characterization of novel cold-sensitive dnaA mutants of Escherichia coli. FEMS Microbiol. Lett. 176, 357-366
  232. Akimitsu, N., Hamamoto, H., Inoue, R., Shoji, M., Akamine, A., Takemori, K., Hamasaki, N., and Sekimizu, K. (1999) Increase in resistance of methicillin-resistant Staphylococcus aureus to beta-lactams caused by mutations conferring resistance to benzalkonium chloride, a disinfectant widely used in hospitals. Antimicrob. Agents Chemother. 43, 3042-3043
  233. Ohba, A., Inoue, R., Akimitsu, N., Mizushima, T., and Sekimizu, K. (1998) Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli. Biol. Pharm. Bull. 21, 1139-1141
  234. Mizushima, T., Takaki, T., Kubota, T., Tsuchiya, T., Miki, T., Katayama, T., and Sekimizu, K. (1998) Site-directed mutational analysis for the ATP binding of DnaA protein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP-binding domain of DnaA protein in vitro and in vivo. J. Biol. Chem. 273, 20847-20851
  235. Kurokawa, K., Mizushima, T., Kubota, T., Tsuchiya, T., Katayama, T., and Sekimizu, K. (1998) A stimulation factor for hydrolysis of ATP bound to DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli. Biochem. Biophys. Res. Commun. 243, 90-95
  236. Katayama, T., Kubota, T., Kurokawa, K., Crooke, E., and Sekimizu, K. (1998) The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E. coli chromosomal replicase. Cell 94, 61-71
  237. Hase, M., Ishikawa, Y., Sekimizu, K., Tsuchiya, T., and Mizushima, T. (1998) Effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. J. Biochem. 123, 680-683
  238. Suzuki, E., Mizushima, T., and Sekimizu, K. (1997) Alteration of fatty acid composition in a pgsA3 mutant of Escherichia coli. Biol. Pharm. Bull. 20, 479-481
  239. Shah, G. H., Kataoka, K., Ueno, A., Ohtsuka, Y., Mizushima, T., and Sekimizu, K. (1997) Psychotropic drugs and local anesthetics restore cardiolipin-inhibited mammalian DNA topoisomerase I activity. Biol. Pharm. Bull. 20, 1233-1234
  240. Ohtsuka, Y., Mizushima, T., Miki, T., and Sekimizu, K. (1997) Transient DNA relaxation in Escherichia coli induced by nalidixic acid. Biol. Pharm. Bull. 20, 467-470
  241. Ohba, A., Mizushima, T., Katayama, T., and Sekimizu, K. (1997) Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. FEBS Lett. 404, 125-128
  242. Ogata, Y., Inoue, R., Mizushima, T., Kano, Y., Miki, T., and Sekimizu, K. (1997) Heat shock-induced excessive relaxation of DNA in Escherichia coli mutants lacking the histone-like protein HU. Biochim. Biophys. Acta 1353, 298-306
  243. Nishida, S., Mizushima, T., Miki, T., and Sekimizu, K. (1997) Immotile phenotype of an Escherichia coli mutant lacking the histone-like protein HU. FEMS Microbiol. Lett. 150, 297-301
  244. Mizushima, T., Yokoyama, K., Mima, S., Tsuchiya, T., and Sekimizu, K. (1997) Inhibition of thymidine transport in dnaA mutants of Escherichia coli. J. Biol. Chem. 272, 21195-21200
  245. Mizushima, T., Nishida, S., Kurokawa, K., Katayama, T., Miki, T., and Sekimizu, K. (1997) Negative control of DNA replication by hydrolysis of ATP bound to DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli. EMBO J. 16, 3724-3730
  246. Mizushima, T., Matsuo, M., and Sekimizu, K. (1997) Induction of DnaK and GroEL heat shock proteins by fluoroquinolones in Escherichia coli. Antimicrob. Agents Chemother. 41, 193-195
  247. Mizushima, T., Koyanagi, R., Katayama, T., Miki, T., and Sekimizu, K. (1997) Decrease in expression of the master operon of flagellin synthesis in a dnaA46 mutant of Escherichia coli. Biol. Pharm. Bull. 20, 327-331
  248. Mizushima, T., Kataoka, K., Ogata, Y., Inoue, R., and Sekimizu, K. (1997) Increase in negative supercoiling of plasmid DNA in Escherichia coli exposed to cold shock. Mol. Microbiol. 23, 381-386
  249. Kubota, T., Katayama, T., Ito, Y., Mizushima, T., and Sekimizu, K. (1997) Conformational transition of DnaA protein by ATP: structural analysis of DnaA protein, the initiator of Escherichia coli chromosome replication. Biochem. Biophys. Res. Commun. 232, 130-135
  250. Katayama, T., Takata, M., and Sekimizu, K. (1997) CedA is a novel Escherichia coli protein that activates the cell division inhibited by chromosomal DNA over-replication. Mol. Microbiol. 26, 687-697
  251. Katayama, T., Akimitsu, N., Mizushima, T., Miki, T., and Sekimizu, K. (1997) Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product. Mol. Microbiol. 25, 661-670
  252. Katayama, H., Mizushima, T., Miki, T., and Sekimizu, K. (1997) Molecular cloning and sequence analysis of the dnaA gene of Staphylococcus aureus. Biol. Pharm. Bull. 20, 820-822
  253. Saruta, F., Hirawake, H., Takamiya, S., Ma, Y. C., Aoki, T., Sekimizu, K., Kojima, S., and Kita, K. (1996) Cloning of a cDNA encoding the small subunit of cytochrome b558 (cybS) of mitochondrial fumarate reductase (complex II) from adult Ascaris suum. Biochim. Biophys. Acta 1276, 1-5
  254. Ogata, Y., Mizushima, T., Kataoka, K., Kita, K., Miki, T., and Sekimizu, K. (1996) DnaK heat shock protein of Escherichia coli maintains the negative supercoiling of DNA against thermal stress. J. Biol. Chem. 271, 29407-29414
  255. Murayama, N., Shimizu, H., Takiguchi, S., Baba, Y., Amino, H., Horiuchi, T., Sekimizu, K., and Miki, T. (1996) Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F. J. Mol. Biol. 256, 483-502
  256. Mizushima, T., Tanino, J., Miki, T., and Sekimizu, K. (1996) Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli. Biochem. Biophys. Res. Commun. 218, 137-141
  257. Mizushima, T., Shinpuku, T., Katayama, H., Kataoka, K., Guo, L., Miki, T., and Sekimizu, K. (1996) Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives. Mol. Gen. Genet. 252, 212-215
  258. Mizushima, T., Sasaki, S., Ohishi, H., Kobayashi, M., Katayama, T., Miki, T., Maeda, M., and Sekimizu, K. (1996) Molecular design of inhibitors of in vitro oriC DNA replication based on the potential to block the ATP binding of DnaA protein. J. Biol. Chem. 271, 25178-25183
  259. Mizushima, T., Ohtsuka, Y., Mori, H., Miki, T., and Sekimizu, K. (1996) Increase in synthesis and stability of sigma 32 on treatment with inhibitors of DNA gyrase in Escherichia coli. Mol. Gen. Genet. 253, 297-302
  260. Mizushima, T., Katayama, T., and Sekimizu, K. (1996) Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli. Biochemistry 35, 11512-11516
  261. Mizushima, T., Ishikawa, Y., Obana, E., Hase, M., Kubota, T., Katayama, T., Kunitake, T., Watanabe, E., and Sekimizu, K. (1996) Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein. J. Biol. Chem. 271, 3633-3638
  262. Matsuo, M., Ohtsuka, Y., Kataoka, K., Mizushima, T., and Sekimizu, K. (1996) Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones. J. Pharm. Pharmacol. 48, 985-987
  263. Maki, S., Takiguchi, S., Horiuchi, T., Sekimizu, K., and Miki, T. (1996) Partner switching mechanisms in inactivation and rejuvenation of Escherichia coli DNA gyrase by F plasmid proteins LetD (CcdB) and LetA (CcdA). J. Mol. Biol. 256, 473-482
  264. Kurokawa, K., Mizushima, T., Miki, T., and Sekimizu, K. (1996) Influence of the linking number of template DNA on sigma 32-dependent transcription initiation in vitro of the Escherichia coli groE gene. Biol. Pharm. Bull. 19, 922-931
  265. Katayama, T., Takata, M., and Sekimizu, K. (1996) The nucleoid protein H-NS facilitates chromosome DNA replication in Escherichia coli dnaA mutants. J. Bacteriol. 178, 5790-5792
  266. Katayama, T., Kubota, T., Takata, M., Akimitsu, N., and Sekimizu, K. (1996) Disruption of the hslU gene, which encodes an ATPase subunit of the eukaryotic 26S proteasome homolog in Escherichia coli, suppresses the temperature-sensitive dnaA46 mutation. Biochem. Biophys. Res. Commun. 229, 219-224
  267. Kataoka, K., Mizushima, T., Ogata, Y., Miki, T., and Sekimizu, K. (1996) Heat shock-induced DNA relaxation in vitro by DNA gyrase of Escherichia coli in the presence of ATP. J. Biol. Chem. 271, 24806-24810
  268. Kaneko, T., Mizushima, T., Ohtsuka, Y., Kurokawa, K., Kataoka, K., Miki, T., and Sekimizu, K. (1996) Co-induction of DNA relaxation and synthesis of DnaK and GroEL proteins in Escherichia coli by expression of LetD (CcdB) protein, an inhibitor of DNA gyrase encoded by the F factor. Mol. Gen. Genet. 250, 593-600
  269. Akimitsu, N., Mizushima, T., Suzuki, E., Miki, T., and Sekimizu, K. (1996) Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. Biol. Pharm. Bull. 19, 1275-1278
  270. Takeda, S., Kita, K., Miyazaki, H., Natori, S., and Sekimizu, K. (1995) Purification of low-molecular-weight GTP-binding proteins structurally related to MTG33, a mitochondrial GTP-binding protein of Ehrlich ascites tumor cells. J. Biochem. 118, 791-795
  271. Shinpuku, T., Mizushima, T., Guo, L., Miki, T., and Sekimizu, K. (1995) Phenotypes of dnaA mutants of Escherichia coli sensitive to detergents and organic solvents. Biochem. Biophys. Res. Commun. 212, 84-89
  272. Saruta, F., Kuramochi, T., Nakamura, K., Takamiya, S., Yu, Y., Aoki, T., Sekimizu, K., Kojima, S., and Kita, K. (1995) Stage-specific isoforms of complex II (succinate-ubiquinone oxidoreductase) in mitochondria from the parasitic nematode, Ascaris suum. J. Biol. Chem. 270, 928-932
  273. Sagara, Y., Matsunaga, K., Nakamura, K., Aramaki, H., Watanabe, Y., Hara, T., Sekimizu, K., and Horiuchi, T. (1995) Purification and characterization of rat adrenodoxin. Biochimie 77, 719-723
  274. Mizushima, T., Koyanagi, R., Suzuki, E., Tomura, A., Kutsukake, K., Miki, T., and Sekimizu, K. (1995) Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli. Biochim. Biophys. Acta 1245, 397-401
  275. Katayama, T., Crooke, E., and Sekimizu, K. (1995) Characterization of Escherichia coli DnaAcos protein in replication systems reconstituted with highly purified proteins. Mol. Microbiol. 18, 813-820
  276. Hase, M., Mizushima, T., Katayama, T., and Sekimizu, K. (1995) Isolation and characterization of inhibitory factors of DNA polymerase III holoenzyme from Escherichia coli. FEMS Microbiol. Lett. 130, 215-220
  277. Yasukochi, T., Okada, O., Hara, T., Sagara, Y., Sekimizu, K., and Horiuchi, T. (1994) Putative functions of phenylalanine-350 of Pseudomonas putida cytochrome P-450cam. Biochim. Biophys. Acta 1204, 84-90
  278. Xu, Q., Nakanishi, T., Sekimizu, K., and Natori, S. (1994) Cloning and identification of testis-specific transcription elongation factor S-II. J. Biol. Chem. 269, 3100-3103
  279. Ogata, Y., Mizushima, T., Kataoka, K., Miki, T., and Sekimizu, K. (1994) Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli. Mol. Gen. Genet. 244, 451-455
  280. Nakamura, K., Horiuchi, T., Yasukochi, T., Sekimizu, K., Hara, T., and Sagara, Y. (1994) Significant contribution of arginine-112 and its positive charge of Pseudomonas putida cytochrome P-450cam in the electron transport from putidaredoxin. Biochim. Biophys. Acta 1207, 40-48
  281. Mizushima, T., Tomura, A., Shinpuku, T., Miki, T., and Sekimizu, K. (1994) Loss of flagellation in dnaA mutants of Escherichia coli. J. Bacteriol. 176, 5544-5546
  282. Mizushima, T., Ohtsuka, Y., Miki, T., and Sekimizu, K. (1994) Temperature shift-up leads to simultaneous and continuous plasmid DNA relaxation and induction of DnaK and GroEL proteins in anaerobically growing Escherichia coli cells. FEMS Microbiol. Lett. 121, 333-336
  283. Tomura, A., Ishikawa, T., Sagara, Y., Miki, T., and Sekimizu, K. (1993) Requirement of phosphatidylglycerol for flagellation of Escherichia coli. FEBS Lett. 329, 287-290
  284. Takeda, S., Sagara, Y., Kita, K., Natori, S., and Sekimizu, K. (1993) Purification of a GTP-binding protein localized in mitochondria. J. Biochem. 114, 684-690
  285. Takata, Y., Sagara, Y., Kono, A., Sekimizu, K., and Horiuchi, T. (1993) Gene structure of bovine adrenodoxin reductase. Biol. Pharm. Bull. 16, 1200-1206
  286. Sagara, Y., Wada, A., Takata, Y., Waterman, M. R., Sekimizu, K., and Horiuchi, T. (1993) Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization. Biol. Pharm. Bull. 16, 627-630
  287. Ogata, Y., Miki, T., and Sekimizu, K. (1993) A role of heat shock proteins for homologous recombination in Escherichia coli. Biochem. Biophys. Res. Commun. 197, 34-39
  288. Mizushima, T., Natori, S., and Sekimizu, K. (1993) Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli. Mol. Gen. Genet. 238, 1-5
  289. Mizushima, T., Natori, S., and Sekimizu, K. (1993) Inhibition of DNA synthesis in Meth A cells by chlorpromazine. Biol. Pharm. Bull. 16, 953-955
  290. Koga, H., Sagara, Y., Yaoi, T., Tsujimura, M., Nakamura, K., Sekimizu, K., Makino, R., Shimada, H., Ishimura, Y., Yura, K., and et al. (1993) Essential role of the Arg112 residue of cytochrome P450cam for electron transfer from reduced putidaredoxin. FEBS Lett. 331, 109-113
  291. Hirai, H., Takeda, S., Natori, S., and Sekimizu, K. (1993) Inhibition of SV40 DNA replication in vitro by chlorpromazine. Biol. Pharm. Bull. 16, 565-567
  292. Haq, S., Natori, S., and Sekimizu, K. (1993) Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in the presence of ethidium bromide. J. Biochem. 113, 620-624
  293. Tanji, K., Ohta, Y., Kawato, S., Mizushima, T., Natori, S., and Sekimizu, K. (1992) Decrease by psychotropic drugs and local anaesthetics of membrane fluidity measured by fluorescence anisotropy in Escherichia coli. J. Pharm. Pharmacol. 44, 1036-1037
  294. Tanji, K., Mizushima, T., Natori, S., and Sekimizu, K. (1992) Induction by psychotropic drugs and local anesthetics of DnaK and GroEL proteins in Escherichia coli. Biochim. Biophys. Acta 1129, 172-176
  295. Nakanishi, T., Nakano, A., Nomura, K., Sekimizu, K., and Natori, S. (1992) Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae. J. Biol. Chem. 267, 13200-13204
  296. Mizushima, T., Natori, S., and Sekimizu, K. (1992) Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids. Biochem. J. 285 ( Pt 2), 503-506
  297. Hirai, H., Natori, S., and Sekimizu, K. (1992) Reversal by phosphatidylglycerol and cardiolipin of inhibition of transcription and replication by histones in vitro. Arch. Biochem. Biophys. 298, 458-463
  298. Kanai, A., Kuzuhara, T., Sekimizu, K., and Natori, S. (1991) Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA. J. Biochem. 109, 674-677
  299. Hirai, H., Takahashi, N., Natori, S., and Sekimizu, K. (1991) Binding of simian virus 40 large tumor antigen to phospholipid vesicles. Biochim. Biophys. Acta 1090, 305-310
  300. Hirai, H., Natori, S., and Sekimizu, K. (1991) Inhibitory action of phospholipid-interacting drugs on transcription initiation in a nuclear extract of Ehrlich ascites tumor cells. Biochim. Biophys. Acta 1088, 191-196
  301. Tamura, H., Ikegami, Y., Ono, K., Sekimizu, K., and Andoh, T. (1990) Acidic phospholipids directly inhibit DNA binding of mammalian DNA topoisomerase I. FEBS Lett. 261, 151-154
  302. Horikoshi, N., Sekimizu, K., and Natori, S. (1990) Site-directed mutagenesis of arginine 246, glutamic acid 247, and histidine 248 in the eukaryotic transcription factor S-II. J. Biol. Chem. 265, 11854-11857
  303. Takeda, S., Sugiyama, H., Natori, S., and Sekimizu, K. (1989) Nuclear GTP-binding proteins of Swiss 3T3 cells. FEBS Lett. 244, 469-472
  304. Lee, D. I., Hirai, H., Natori, S., and Sekimizu, K. (1989) Restricted degradation of U6 RNA in a nuclear extract of Ehrlich ascites tumor cells. J. Biochem. 105, 526-528
  305. Hirashima, S., Horikoshi, N., Sekimizu, K., and Natori, S. (1989) Production of functional S-II in Bombyx mori cells. Biochem. Biophys. Res. Commun. 160, 1093-1099
  306. Sekimizu, K., Yung, B. Y., and Kornberg, A. (1988) The dnaA protein of Escherichia coli. Abundance, improved purification, and membrane binding. J. Biol. Chem. 263, 7136-7140
  307. Sekimizu, K., and Kornberg, A. (1988) Cardiolipin activation of dnaA protein, the initiation protein of replication in Escherichia coli. J. Biol. Chem. 263, 7131-7135
  308. Sekimizu, K., Bramhill, D., and Kornberg, A. (1988) Sequential early stages in the in vitro initiation of replication at the origin of the Escherichia coli chromosome. J. Biol. Chem. 263, 7124-7130
  309. Ohtsuki, M., Nakanishi, Y., Sekimizu, K., and Natori, S. (1988) Difference between supercoiled and linear deoxyribonucleic acids in preinitiation complex formation for accurate transcription in vitro. Chem. Pharm. Bull. (Tokyo) 36, 2517-2522
  310. Hirai, H., Sekimizu, K., Horikoshi, M., Nakanishi, Y., and Natori, S. (1988) Stimulation of transcription from accurate initiation sites by purified S-II. FEBS Lett. 238, 119-122
  311. Hirai, H., Lee, D. I., Natori, S., and Sekimizu, K. (1988) Uridylation of U6 RNA in a nuclear extract in Ehrlich ascites tumor cells. J. Biochem. 104, 991-994
  312. Sekimizu, K., Bramhill, D., and Kornberg, A. (1987) ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome. Cell 50, 259-265
  313. Hirashima, S., Horikoshi, M., Nakanishi, Y., Hirai, H., Sekimizu, K., Mizuno, D., and Natori, S. (1987) A rapid and simple procedure for purification of S-II, a transcription factor of ribonucleic acid polymerase II. Chem. Pharm. Bull. (Tokyo) 35, 877-881
  314. Shiraishi, A., Nakanishi, Y., Sekimizu, K., and Natori, S. (1986) Analysis of the DNase I-hypersensitive site of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina. J. Biol. Chem. 261, 940-943
  315. Baker, T. A., Sekimizu, K., Funnell, B. E., and Kornberg, A. (1986) Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome. Cell 45, 53-64
  316. Sekimizu, K., and Natori, S. (1985) Immunological analysis of eukaryotic transcription factor S-II in a HeLa cell lysate. Biochem. Biophys. Res. Commun. 127, 956-961
  317. Matsumoto, N., Sekimizu, K., Soma, G., Ohmura, Y., Andoh, T., Nakanishi, Y., Obinata, M., and Natori, S. (1985) Structural analysis of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina. J. Biochem. 97, 1501-1508
  318. Horikoshi, M., Sekimizu, K., and Natori, S. (1985) Conformational change of DNA binding subunit of RNA polymerase II on binding to DNA. Biochem. Biophys. Res. Commun. 129, 141-147
  319. Horikoshi, M., Sekimizu, K., Hirashima, S., Mitsuhashi, Y., and Natori, S. (1985) Structural relationships of the three stimulatory factors of RNA polymerase II from Ehrlich ascites tumor cells. J. Biol. Chem. 260, 5739-5744
  320. Hirashima, S., Nakanishi, Y., Sekimizu, K., and Natori, S. (1985) Phosphorylation of S-II is not affected by inhibitors of RNA synthesis. Biochem. Biophys. Res. Commun. 131, 524-531
  321. Hayashi, H., Nishimura, Y., Tsuboi, M., Sekimizu, K., Nakanishi, Y., and Natori, S. (1985) Raman spectrum of a closed-circular DNA. Biopolymers 24, 1107-1111
  322. Sekimizu, K., Horikoshi, M., and Natori, S. (1984) Enhancement of the frequency of initiation by a stimulatory protein of RNA polymerase II. J. Biochem. 96, 1859-1865
  323. Ohtsuki, M., Sekimizu, K., and Natori, S. (1984) Accurate transcription initiation in an Ehrlich ascites tumor cell lysate. J. Biochem. 96, 1267-1272
  324. Ohtsuki, M., Sekimizu, K., Agemori, M., Shizuta, Y., and Natori, S. (1984) Effect of the DNA-binding domain of poly(ADP-ribose)synthetase on accurate transcription initiation in a HeLa cell lysate. FEBS Lett. 168, 275-277
  325. Horikoshi, M., Sekimizu, K., and Natori, S. (1984) Analysis of the stimulatory factor of RNA polymerase II in the initiation and elongation complex. J. Biol. Chem. 259, 608-611
  326. Sekimizu, K., Horikoshi, M., Okajima, H., and Natori, S. (1983) Identification of subunits of RNA polymerase II from Ehrlich ascites tumor cells. J. Biochem. 93, 1523-1530
  327. Matsumoto, N., Sekimizu, K., Horikoshi, M., Ohtsuki, M., Kidani, Y., and Natori, S. (1983) Preferential Inhibition of the Activity of a Stimulatory Protein of Eukaryotic Transcription by Platinum(II) Complexes. Cancer Res. 43, 4338-4342
  328. Horikoshi, M., Tamura, H., Sekimizu, K., Obinata, M., and Natori, S. (1983) Identification of the DNA binding subunit of RNA polymerase II from Ehrlich ascites tumor cells. J. Biochem. 94, 1761-1767
  329. Sekimizu, K., Yokoi, H., and Natori, S. (1982) Evidence that stimulatory factor(s) of RNA polymerase II participates in accurate transcription in a HeLa cell lysate. J. Biol. Chem. 257, 2719-2721
  330. Ueno, K., Sekimizu, K., Obinata, M., Mizuno, D., and Natori, S. (1981) Stimulation of messenger ribonucleic acid synthesis in isolated nuclei by a protein that stimulates RNA polymerase II. Biochemistry 20, 634-640
  331. Sekimizu, K., Kubo, Y., Segawa, K., and Natori, S. (1981) Difference in phosphorylation of two factors stimulating RNA polymerase II of Ehrlich ascites tumor cells. Biochemistry 20, 2286-2292
  332. Nakanishi, Y., Sekimizu, K., Tamura, H., and Natori, S. (1981) Purification of a new protein stimulating RNA polymerase II from Ehrlich ascites tumor cells: comparison with proteins purified before. J. Biochem. 90, 805-814
  333. Nakanishi, Y., Mitsuhashi, Y., Sekimizu, K., Yokoi, H., Tanaka, Y., Horikoshi, M., and Natori, S. (1981) Characterization of three proteins stimulating RNA polymerase II. FEBS Lett. 130, 69-72
  334. Tamura, H., Sekimizu, K., Obinata, M., and Natori, S. (1980) Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test. J. Biochem. 88, 1475-1480
  335. Ueno, K., Sekimizu, K., Mizuno, D., and Natori, S. (1979) Antibody against a stimulatory factor of RNA polymerase II inhibits nuclear RNA synthesis. Nature 277, 145-146
  336. Sekimizu, K., Nakanishi, Y., Mizuno, D., and Natori, S. (1979) Purification and preparation of antibody to RNA polymerase II stimulatory factors from Ehrlich ascites tumor cells. Biochemistry 18, 1582-1588
  337. Sekimizu, K., Mizuno, D., and Natori, S. (1979) Localization of a factor stimulating RNA polymerase II in the nucleoplasm, revealed by immunofluorescence. Exp. Cell Res. 124, 63-72
  338. Nakanishi, Y., Sekimizu, K., Mizuno, D., and Natori, S. (1978) Apparent difference in the way of RNA synthesis stimulation by two stimulatory factors of RNA polymerase II. FEBS Lett. 93, 357-360
  339. Sekimizu, K., Mizuno, D., and Natori, S. (1977) Enhancement of ormation of the initiation complex by factor stimulating RNA polymerase II from Ehrlich ascites tumor cells. Biochim. Biophys. Acta 479, 180-187
  340. Kuroiwa, A., Sekimizu, K., Ueno, K., Mizuno, D., and Natori, S. (1977) Separation of a stimulatory factor of RNA polymerase II from protein kinase activity of Ehrlich ascites tumor cells. FEBS Lett. 75, 183-186
  341. Sekimizu, K., Kobayashi, N., Mizuno, D., and Natori, S. (1976) Purification of a factor from Ehrlich ascites tumor cells specifically stimulating RNA polymerase II. Biochemistry 15, 5064-5070
  342. Natori, S., Sekimizu, K., and Mizuno, D. (1974) Organ specificity and sensitivity to alkaline phosphatase of factors stimulating RNA polymerase II. J. Biochem. 76, 695-702